Introduction

Cytometry

The objective of the Cytometry laboratory is to support initiatives from either university personnel or other organisations and companies which request the use of cytometrical techniques (cellular analysis by means of flow cytometry, cellular separation by means of Fluorescence Activated Cell Sorting or nucleic acid hybridization techniques).

Main Work Areas

Cellular analysis by means of flow cytometry

The flow cytometry is based on the fast, simultaneous study of various parameters of optical nature over a great number of particles susceptible to be marked with a fluorescent substance. For this purpose, the particles (complete cells, nucleus, or microorganisms from 0.5-30 micron) must be individually suspended and be susceptible to be marked with at least one fluorochrome simultaneously. Besides, from each analysed particle, information regarding its size and structural complexity is collected. This allows for the analysis of subpopulations in complex samples by means of electron separation.

Sorting

It is also based on flow cytometry. The cellular separation by means of FACs allows for the physical isolation of cellular subpopulations. This sorting can be carried out in test tubes or in 6, 24 or 96 well plates and under aseptic conditions if necessary.

In Situ Hybridization Technique

FISH (Fluorescent In Situ Hybridization)

In situ hybridization is a cytochemistry technique (performed on tissue, cells or chromosomes without the need of the previous extraction of nucleic acids) which allows for the detection and localisation of specific DNA or RNA sequences in biological structures on coverslips. The procedure is based on the hybridisation of two complementary nucleic acid sequences where the marked probe makes a hybrid with specific target sequences which are present in the microscopic preparation's cells or chromosomes.

The hybrid which is formed can be seen directly by using a microscope or after the immunocytochemical detection either by means of enzyme or fluorochrome conjugated antibodies. The in situ hybridisation attached to a fluorescence system is called FISH; it is a new technology which utilizes fluorescence marked DNA-probes to detect or confirm genetic or chromosomal anomalies (either numeric or structural) with a superior resolution power compared to the routine cytogenetic.

Sequencing

The objective of the Sequencing laboratory is to support the university research groups as well as other organizations and companies providing them the most advanced equipment commercialized in the DNA analysis field either for sequencing or genotyping.

Furthermore, it offers a technical support service for all non-expert users who are willing to incorporate these techniques in their work field.

Biotechnology and cellular culture

The Biotechnology and cellular culture laboratory facilitates the creation of hybridomas and murine monoclonal antibodies as well as rabbit polyclonal serums which are specific for antigens of interest for researchers.

Besides, cultures of cell lines are also done under the conditions requested by the researchers as well as the cytotoxicity and cellular proliferation testing for the analysis of new drugs and materials.

A cell-line bank is available for the researchers as well as a biological-sample cryopreservation service in freezers at a temperature of -150 °C or in liquid Nitrogen tanks (-196 °C).

Another service offered is the purification of proteins by means of the KTA FPLC equipment.

We also provide tuning and advice on protein electrophoresis and immunotests (western Blot and ELISA).

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The equipment of these facilities is co-financed by the European Regional Development Fund (ERDF), with the management of the Ministry of Economy and Competitiveness.

The equipment of these facilities is co-financed by the European Regional Development Fund (ERDF), with the management of the Ministry of Economy and Competitiveness.